Animals used for fluorescent imaging should be fed a diet free of what substance?

Feeding animals a diet free of chlorophyll is essential for fluorescent imaging because chlorophyll can interfere with the fluorescent signals that are being measured. Fluorescent imaging relies on the ability to detect specific fluorescent markers or dyes that emit light at particular wavelengths. If the animals in the study consume chlorophyll, which has its own fluorescent properties, this can lead to background fluorescence that obscures or complicates the interpretation of the intended fluorescent signals.

By using animals that are free from chlorophyll, researchers can obtain clearer and more accurate readings of the fluorescent markers, leading to more reliable results in the imaging process. In contrast, glucose, protein, and fats do not inherently affect the fluorescent properties of the imaging materials commonly used and are not typically a concern when it comes to ensuring the clarity of fluorescent signals.