What is the primary focus when monitoring a breeding female mouse?

The primary focus when monitoring a breeding female mouse is checking the condition of the nest and pups. This is crucial because the health and well-being of the pups directly influence both their survival and the breeding success of the female. Ensuring that the nest is clean, secure, and maintained at appropriate humidity levels is essential for neonatal survival. Pups are extremely vulnerable in their early days, and any factors affecting their environment can lead to increased mortality or health problems.

While maintaining comfortable temperatures, ensuring adequate nutrition, and observing social interactions with other females are all important aspects of animal care, they are secondary to the immediate needs of the pups at this stage. The nesting environment directly impacts the pups' ability to thrive, making it the top priority. Regular checks help in mitigating potential issues early on, thus promoting a successful breeding program.